High Content Imaging


ImageXpress Micro XLS Widefield High-Content Analysis System


Fast, flexible and robust widefield high-content imaging for fixed- or live-cell assays

The ImageXpress® Micro XLS System is a widefield automated microscope capable of fluorescent, transmitted light, and phase-contrast imaging of fixed- or live-cell assays, tissues and small organisms. Speed, flexibility and quality are assured with 3x field-of-view, industry-leading stage and autofocus control, the broadest range of research-grade objective lenses (1x-100x) available, multiple filter options, and a suite of MetaXpress® Software solutions to facilitate and optimize image analysis. 

  • Fewer images per well and shorter imaging time with a field of view that is 3x larger than industry standard
  • A breadth of applications enabled by objectives ranging from 1x to 100x, 0.05 to 0.95 NA for air and 1.4 NA for oil, capturing a variety of cellular and intra-cellular events
  • Accurate stage positioning for capturing high-resolution tiled or stitched images with fully-automated 100 nm resolution voice coil-driven stages, thereby minimizing X-Y stage jitter and ensuring repeatability of extended live-cell experiments
  • Up to 5x faster read times while minimizing photobleaching with industry-leading autofocus using a dedicated 690 nm focus laser and ultra-fast sensors
  • High-throughput screening captures > 10 million cells/hour in a low-resolution, whole-well, 3 color cell scoring application, or > 1 million cells/hour in a high resolution two-color assay
  • Minimized user intervention using automated 5-position filter cube changer, facilitating multiplex assays with up to five filter sets and four objectives for a single experiment
A 5-color assay achieved with multiple filters. Mouse embryo sections
 Blue = DAPI, Green = FITC, Red = TRITC, Orange = Cy5
  • Modular design allowing for transmitted light, phase contrast, environmental control, fluidics, light source and external filter wheel selection
  • Extensive sample compatibility from slides to plates (6- to 1536-well), thin to thick plate bottoms, glass to plastic, transwell, low profile- and low volume-plates
  • Integrated automation, 24/7 with 3rd party robotic plate handling system, enabling users to schedule multiple runs.