Molecular biology

PCR reagens, Master Mixes, Restriction/Modifying Enzymes, Nucleosides, Nucleotides and their Analogs, Diagnostic kits, Competent Cells, Markers, Buffers and Gels, Macromolecular Crystalization.

Complete Solution for Molecular Biology and Biochemistry.

  • Natural nucleotides and modified analogs
  • Libraries of Nucleotide analogs for screening
  • Modified nucleoside analogs
  • Nucleoside Phosphoramidites
  • Custom Synthesis of exotic nucleotide analogs

 

 

  • DNA, RNA, protein synthesis monitoring
  • Enzymatic CLICK-functionalization of DNA, RNA or proteins
  • Posttranslational Modification Analysis (Phosphorylation, Glycosylation, AMPylation, ADP-Ribosylation...)

 

 

  • Standard PCR and Real-Time PCR reagens and kits
  • Reverse Transcription
  • in vitro Transcription
  • Primers and Oligonucleotides
  • Labelling of DNA/cDNA or RNA/cRNA
  • Cloning and Mutagenesis
  • RNA / DNA Preparation and Cleanup
  • DNA Ladders
  • Protein MW Markers / Blot stain
  • Restriction/Modifying Enzymes

 

  • Reactive Fluorescent, Luminescent and bifunctional reagents for labeling of biomolecules
  • Fluorescent, Hapten-, Click Chemistry- and Amine- modified Nucleotides for the enzymatic preparation of labeled DNA/cDNA or RNA/cRNA probes
  • Novel Classic Labeling Substances and Kits for Fluorescent, Hapten- and Luminescent-labeling of protein (N- and/or C-terminal)
  • Fluorescent reporter and Protein & Nucleotide Internalization Kits

 

 

  • Eukaryotic protein expression in Leishmania tarentolae eukaryotic host
  • Not pathogenic to mammalians (Biosafety level 1)
  • Inducible (Genome integrated), Inducible (Episomal), Constitutive (Genome integrated)
  • Shuttle vectors: cloning in E. coli, expression in LEXSY host

 

 

  • Transcription Factors
  • GTP Signal Transduction
  • Recombinant Viral and Microbial Proteins
  • Cytokines and Growth Factors
  • Amyloid and Prion Proteins
  • Protein Kinases and Deacetylases
  • Native Viral and Microbial Proteins
  • Phosphatases and Proteases
  • Receptors
  • Calcium dependent Proteins
  • Antioxidants
  • Carbonic Anhydrases

 

 

  • Enzyme Assays
  • Protein & nucleic acid Internalization
  • Contamination Control Kits
  • Antibodies
  • Nourseothricin (also termed NTC or clonNAT)
  • Protein Prenylation
  • Fungal Enzymes

 

 

  • Agarose and Magnetic Beads as matrix for different purification tasks
  • Purification of Nucleotide-, mRNA cap-, polyA-, Phospho Amino Acid- binding proteins
  • Purification of proteins tagged GST or His
  • Purification of CLICK-functionalized proteins

 

 

  • Protein Crystallization Kits
  • Screening Tools
  • Process Optimization - Chemical Environment/ Thermodynamics / Kinetics
  • Sample Retrieval, Mounting, Manipulation and Measurement
  • Phasing kits

 

 

 

Bioenergetika,Bioengineering Sacharidů a Molekulární Biologie

 

 

 

 

 

Direct PCR DNA Extraction Reagents for PCR and Genotyping

Viagen DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending reagent developed by scientists at Viagen Biotech Inc. allows the resulting DNA crude extract to be compatible with genomic PCR for genotyping.

Protocols and Detail you can find here